Protective immunity against vibrio cholerae infection in the rabbit
Identifieur interne : 001398 ( Main/Exploration ); précédent : 001397; suivant : 001399Protective immunity against vibrio cholerae infection in the rabbit
Auteurs : Piet A. M. Guinée [Pays-Bas] ; Wim H. Jansen [Pays-Bas] ; Henk Gielen [Pays-Bas] ; Sjoerd G. T. Rijpkema [Pays-Bas] ; Paul W. J. Peters [Pays-Bas]Source :
- Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene / A: Medical Microbiology, Infectious Diseases, Virology, Parasitology [ 0176-6724 ] ; 1987.
Abstract
The DIC model (Duodenal Inoculation with ligation of the Cecum in rabbits) was employed to study experimentally induced cholera and the related protective immunity. Duodenal inoculation (DI) without ligation of the cecum with live V. cholerae organisms did not cause any disease symptom but induced protection against subsequent challenges with homologous and heterologous organisms for up to 24 months. After 30 months this protective immunity began to decrease. A similar protective immunity could be induced by administration of the A— B+ derivative CVD101 of V. cholerae strain 395. This type of experiment can only be done successfully with conventional, healthy rabbits held under low stress conditions. A so-called specific pathogen-free rabbit breed was found to be entirely unsuitable. Duodenal inoculation with heat- or merthiolate-inactivated V. cholorae for a prolonged period of time by means of an intestinal osmotic minipump did not induce protection. Injection of heat-inactivated V. cholerae material into the Peyer's patches sometimes led to protection, suggesting that a thermostable antigen, possibly lipopolysaccharide, is one of the major protective antigens. Duodenal administration of a combination of inactivated V. cholerae serotypes Ogawa and Inaba cells and 1 mg B subunit of the V. cholerae enterotoxin by up to three inoculations protected only 3 out of 12 rabbits against challenge. The results obtained on the rabbit model are discussed in relation to the efficacy of this vaccine in human volunteers and in a recent field test.
Url:
DOI: 10.1016/S0176-6724(87)80237-7
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protective immunity against vibrio cholerae infection in the rabbit</title><author><name sortKey="Guinee, Piet A M" sort="Guinee, Piet A M" uniqKey="Guinee P" first="Piet A. M." last="Guinée">Piet A. M. Guinée</name></author><author><name sortKey="Jansen, Wim H" sort="Jansen, Wim H" uniqKey="Jansen W" first="Wim H." last="Jansen">Wim H. Jansen</name></author><author><name sortKey="Gielen, Henk" sort="Gielen, Henk" uniqKey="Gielen H" first="Henk" last="Gielen">Henk Gielen</name></author><author><name sortKey="Rijpkema, Sjoerd G T" sort="Rijpkema, Sjoerd G T" uniqKey="Rijpkema S" first="Sjoerd G. T." last="Rijpkema">Sjoerd G. T. Rijpkema</name></author><author><name sortKey="Peters, Paul W J" sort="Peters, Paul W J" uniqKey="Peters P" first="Paul W. J." last="Peters">Paul W. J. Peters</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:20E1511722CCAAF346A23032C83A5CDF24FD3E62</idno><date when="1987" year="1987">1987</date><idno type="doi">10.1016/S0176-6724(87)80237-7</idno><idno type="url">https://api.istex.fr/document/20E1511722CCAAF346A23032C83A5CDF24FD3E62/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000132</idno><idno type="wicri:Area/Main/Curation">000131</idno><idno type="wicri:Area/Main/Exploration">001398</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Exploration">001398</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Protective immunity against vibrio cholerae infection in the rabbit</title><author><name sortKey="Guinee, Piet A M" sort="Guinee, Piet A M" uniqKey="Guinee P" first="Piet A. M." last="Guinée">Piet A. M. Guinée</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>National Institute of Public Health and Environmental Hygiene, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Jansen, Wim H" sort="Jansen, Wim H" uniqKey="Jansen W" first="Wim H." last="Jansen">Wim H. Jansen</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>National Institute of Public Health and Environmental Hygiene, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Gielen, Henk" sort="Gielen, Henk" uniqKey="Gielen H" first="Henk" last="Gielen">Henk Gielen</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>National Institute of Public Health and Environmental Hygiene, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Rijpkema, Sjoerd G T" sort="Rijpkema, Sjoerd G T" uniqKey="Rijpkema S" first="Sjoerd G. T." last="Rijpkema">Sjoerd G. T. Rijpkema</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>National Institute of Public Health and Environmental Hygiene, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Peters, Paul W J" sort="Peters, Paul W J" uniqKey="Peters P" first="Paul W. J." last="Peters">Paul W. J. Peters</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>National Institute of Public Health and Environmental Hygiene, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene / A: Medical Microbiology, Infectious Diseases, Virology, Parasitology</title><title level="j" type="abbrev">ZBMHA</title><idno type="ISSN">0176-6724</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987">1987</date><biblScope unit="volume">266</biblScope><biblScope unit="issue">3–4</biblScope><biblScope unit="page" from="552">552</biblScope><biblScope unit="page" to="562">562</biblScope></imprint><idno type="ISSN">0176-6724</idno></series><idno type="istex">20E1511722CCAAF346A23032C83A5CDF24FD3E62</idno><idno type="DOI">10.1016/S0176-6724(87)80237-7</idno><idno type="PII">S0176-6724(87)80237-7</idno><idno type="ArticleID">80237</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0176-6724</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The DIC model (Duodenal Inoculation with ligation of the Cecum in rabbits) was employed to study experimentally induced cholera and the related protective immunity. Duodenal inoculation (DI) without ligation of the cecum with live V. cholerae organisms did not cause any disease symptom but induced protection against subsequent challenges with homologous and heterologous organisms for up to 24 months. After 30 months this protective immunity began to decrease. A similar protective immunity could be induced by administration of the A— B+ derivative CVD101 of V. cholerae strain 395. This type of experiment can only be done successfully with conventional, healthy rabbits held under low stress conditions. A so-called specific pathogen-free rabbit breed was found to be entirely unsuitable. Duodenal inoculation with heat- or merthiolate-inactivated V. cholorae for a prolonged period of time by means of an intestinal osmotic minipump did not induce protection. Injection of heat-inactivated V. cholerae material into the Peyer's patches sometimes led to protection, suggesting that a thermostable antigen, possibly lipopolysaccharide, is one of the major protective antigens. Duodenal administration of a combination of inactivated V. cholerae serotypes Ogawa and Inaba cells and 1 mg B subunit of the V. cholerae enterotoxin by up to three inoculations protected only 3 out of 12 rabbits against challenge. The results obtained on the rabbit model are discussed in relation to the efficacy of this vaccine in human volunteers and in a recent field test.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Guinee, Piet A M" sort="Guinee, Piet A M" uniqKey="Guinee P" first="Piet A. M." last="Guinée">Piet A. M. Guinée</name></noRegion><name sortKey="Gielen, Henk" sort="Gielen, Henk" uniqKey="Gielen H" first="Henk" last="Gielen">Henk Gielen</name><name sortKey="Jansen, Wim H" sort="Jansen, Wim H" uniqKey="Jansen W" first="Wim H." last="Jansen">Wim H. Jansen</name><name sortKey="Peters, Paul W J" sort="Peters, Paul W J" uniqKey="Peters P" first="Paul W. J." last="Peters">Paul W. J. Peters</name><name sortKey="Rijpkema, Sjoerd G T" sort="Rijpkema, Sjoerd G T" uniqKey="Rijpkema S" first="Sjoerd G. T." last="Rijpkema">Sjoerd G. T. Rijpkema</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/SchutzV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001398 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001398 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= SchutzV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:20E1511722CCAAF346A23032C83A5CDF24FD3E62
|texte= Protective immunity against vibrio cholerae infection in the rabbit
}}
| This area was generated with Dilib version V0.6.38. |  |